SGH calculation of Py-GC/MS data

OpenChrom can be used to perform a calculation of the SGH (Syringyl/Guaiacy/p-Hydroxyphenyl) and carbohydrate ratio of a wood sample measured by Py-GC/MS technique.

Open the Py-GC/MS file via the “Chromatogram File Explorer (MSD)“.

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Then detect and integrate peaks. You could alternatively use deconvolution techniques like AMDIS. In this case, we use the “First Derivative” peak detector.

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The detected peaks are marked with an inverted triangle.

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Please apply a peak integrator then, in this case the “Integrator Trapezoid“.

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The areas are listed in the “Peak List” view.

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Each peak needs to be identified now. Please tun the “Base Peak Identifier” on all selected peaks.

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Each peak is identified as

  • Syringyl
  • Guaiacyl
  • p-Hydroxyphenyl
  • Carbohydrate
  • Not Found

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The run the base peak classifier to calculate the SGH ratio.

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The percentage values are displayed in the “BasePeak Classifier” view.

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