ISTD Quantitation

It’s quite easy to use internal standards (ISTD) for the quantitation of chromatograms in OpenChrom. The first step is to open a chromatogram, detect and integrate peaks. Please open the “Chromatogram Peaks” perspective then.


Please select the view “Peak ISTD (MSD)” and then select a peak. By clicking on the (+) button, the peak can be flagged with the internal standard information.


In this case, the selected peak represents a concentration of 12.0 mg/g.


It’s possible to set several internal standards.


The next step is to quantitate one or all peaks of the chromatogram selection. Use the menu entry “Chromatogram > Quanitifier > Peak Quantifier > Internal Standards (ISTD) > Quantify All Peaks“.


The results for each peak will be displayed in the “Peak Quantitation Entries (MSD)” view.


The view will be updated as soon as another peak is selected.


All quantitation results are saved when using the *.ocb chromatogram format. This enables to review the data whenever you want. Additionally, OpenChrom offers a report to export the data to text format. Use the menu entry “Chromatogram > Reports > OpenChrom Report“.


The data is exported in the following format:

[#]    Name    ChemicalClass    Concentration    ConcentrationUnit    Area    Description    Ion

[12]    Acetic Acid    Acids    2.90922    mg/g    5433521.52496        TIC
[12]    Furfural        2.34891    ppm    5433521.52496        TIC

[13]    Acetic Acid    Acids    12.92843    mg/g    24146306.8746        TIC
[13]    Furfural        10.43846    ppm    24146306.8746        TIC

See, e.g. the following (PDF) snippet: ap-1413


1 Comment

Leave a Reply

Fill in your details below or click an icon to log in: Logo

You are commenting using your account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s