It’s quite easy to use internal standards (ISTD) for the quantitation of chromatograms in OpenChrom. The first step is to open a chromatogram, detect and integrate peaks. Please open the “Chromatogram Peaks” perspective then.
Please select the view “Peak ISTD (MSD)” and then select a peak. By clicking on the (+) button, the peak can be flagged with the internal standard information.
In this case, the selected peak represents a concentration of 12.0 mg/g.
It’s possible to set several internal standards.
The next step is to quantitate one or all peaks of the chromatogram selection. Use the menu entry “Chromatogram > Quanitifier > Peak Quantifier > Internal Standards (ISTD) > Quantify All Peaks“.
The results for each peak will be displayed in the “Peak Quantitation Entries (MSD)” view.
The view will be updated as soon as another peak is selected.
All quantitation results are saved when using the *.ocb chromatogram format. This enables to review the data whenever you want. Additionally, OpenChrom offers a report to export the data to text format. Use the menu entry “Chromatogram > Reports > OpenChrom Report“.
The data is exported in the following format:
PEAK QUANTITATION RESULTS
[#] Name ChemicalClass Concentration ConcentrationUnit Area Description Ion
 Acetic Acid Acids 2.90922 mg/g 5433521.52496 TIC
 Furfural 2.34891 ppm 5433521.52496 TIC
 Acetic Acid Acids 12.92843 mg/g 24146306.8746 TIC
 Furfural 10.43846 ppm 24146306.8746 TIC
See, e.g. the following (PDF) snippet: ap-1413